Step 1: Input query sequences (each sequence should be less than 5000 bps)
The user has three ways to upload the query sequences in the FASTA format:
(i) paste the query sequences into the text area or upload a local file; or
(ii) let DOOR2 prepare the promoter sequences, if they focus on bacteria; or
(iii) let Database of Eukaryotic Genes prepare the promoter sequences, if they focus on eukaryotes.

Step 2: Include control sequences (optional)
It is optional and allows the user to include a set of background sequences as a control. The format and submission requirement of background sequences is the same as the query sequences.

Step 3: Set algorithm parameters (optional)
Set the minimal and maximal motif length, as well as the number of output motifs.

Step 4: Submit job
Before submitting the job, the user can leave their email, which will be contacted when the job is done. To protect DMINDA 2.0 from spam and abuse, a single click on "I'm not a robot" is required.